Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. As a naturopathic doctor and a mom, Tylenol is a hard no in my house and I want to explain why. , , Acetaminophen (Tylenol) is processed through the liver, and in that process it can lower Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Interaction with Ethanol Alcohol Metabolism About Acetaminophen Overdose Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram