5 Oxoprolinuria as a Cause for Metabolic Acidosis Renal Fellow Network Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Let's talk Tylenol Paracetamol (acetaminophen) There are many side effects that most people don't realize. OTC does not mean it is side effect free, especially with chronic use. Even just 4 days of About Acetaminophen Overdose Acetaminophen, a Therapeutic or an Extremely Toxic RemedyA Review